![]() S2 Fig: Enhanced accumulation of an RNAi-deficient Tobacco rattle virus (TRV) RNA1 replicon following co-expression with PPR3 in leaves of Nicotiana benthamiana. The amplification product in lane 4 was expected and is of the expected size (~770 bp). The RNA samples analyzed were: lanes 1 and 4, gfp mRNA lanes 2 and 5, RNA not subjected to reverse transcription lanes 3 and 6, antisense gfp mRNA. Bacteria analyzed with the three primer pairs in lanes 1–3 were transformed with pET24b + empty (no insert a negative control), whereas bacteria analyzed in lanes 4–6 were transformed with pET24b +GFPopt for expression of gfp mRNA. GFP fluorescence in “sense GFP” treatment was significantly lower (unpaired t-test p < 0.001) than in the two other treatments that did not differ from each other. ( B) Comparison of the normalized average GFP fluorescence intensity 72 h post-feeding with bacteria in three independent experiments. Controls included feeding with bacteria harboring an empty (no insert) plasmid or a plasmid lacking the T7 promoter. coli strain expressing gfp mRNA (sense GFP). gfp silencing was induced by feeding the animals an E. elegans intestine taken with bright field (BF) illumination to observe morphology or UV light to observe GFP fluorescence. elegans strain RT476, which expresses gfp under the intestine-specific promoter vha-6. ![]() S1 Fig: Sense-mediated silencing of gfp expression in the gfp-transgenic C. ![]()
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